Lineage Depletion Antibodies & Kits
Lineage depletion antibodies and kits can be used to enrich for uncommitted cell populations.
R&D Systems offers a range of lineage marker antibodies optimized to sufficiently bind 1 x 109 bone marrow derived cells. These antibodies can conveniently be used in conjunction with magnetic particle separation systems (Catalog # MAG997) or flow cytometric cell sorting to deplete lineage-committed cells for the enrichment of mouse uncommitted mesenchymal or hematopoietic stem cells.
| Analyte | Type | Clone # | Catalog # |
| Mouse B220/CD45R | Monoclonal Rat IgG2B | RA3-6B2 | MAB1217 |
| Mouse CD3 | Monoclonal Rat IgG2B | 17A2 | MAB4841 |
| Mouse CD4 | Monoclonal Rat IgG2B | GK1.5 | MAB554 |
| Mouse CD5 | Monoclonal Rat IgG2B | 53-7.3 | MAB115 |
| Mouse CD8 alpha | Monoclonal Rat IgG2B | 53-6.7 | MAB116 |
| Mouse Integrin alpha M/CD11b | Monoclonal Rat IgG2B | M1/70 | MAB1124 |
| Mouse Gr-1/Ly-6G | Monoclonal Rat IgG2B | RB6-8C5 | MAB1037 |
| Mouse TER-119 | Monoclonal Rat IgG2B | TER-119 | MAB1125 |
MagCellect™ Mouse Hematopoietic Cell Lineage Depletion Kit** (Catalog # MAGM209)
Designed for the enrichment of hematopoietic non-lineage committed cells (lineage negative) from mouse bone marrow. Lineage committed cells (lineage positive) targeted for depletion include T cells, B cells, NK cells, monocytes/macrophages, granulocytes, and erythrocytes. The resulting cell population is highly enriched for SCF R/c-kit/CD117+ cells to a purity of 40-70% (depending on mouse strain) with less than 5% residual lineage positive cells. This kit is for use with the MagCellect Magnet; sold separately (Catalog # MAG997)
Kit Contents
- 10X Buffer
- Streptavidin Ferrofluid
- Blocking Reagent
- Anti-CD5
- Anti-TER-119
- Anti-CD11b
- Anti-CD45R/B220
- Anti-Ly-6G/Gr-1
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| Hematopoietic Lineage Depletion. Lineage marker reactivity on BALB/c bone marrow (BM) cells processed with the MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit (Catalog # MAGM209). Histograms show reactivity of BM cells labeled with the cocktail of biotinylated antibodies included in the kit both before (green histogram) and after (open histogram) magnetic depletion. Lineage marker reactivity was detected using Streptavidin-PE. | Enrichment of CD117+ Cells. CD117 (c-kit) staining of bone marrow lineage negative cells isolated from BALB/c mice using the MagCellect Mouse Hematopoietic Cell Lineage Depletion Kit (Catalog # MAGM209). Histograms reflect reactivity of all viable cells with a PE-conjugated Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (Catalog # FAB1356P; light green) or a matched isotype control antibody (open histogram). Inset shows CD117 staining of cells before depletion. |
Mouse Hematopoietic Cell Lineage Depletion Protocol
**This product utilizes and/or contains technology licensed from Veridex, LLC, Raritan, New Jersey 08869, USA, which is covered by one or more claims of United States and International patents and/or pending patent applications.